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Basic Image Processing
Practical Course on using the Fiji software

In this course, students learn how to use ImageJ functions (in the Fiji configuration) to prepare publication-ready material starting from raw microscopy images or image series. The course is given in English and is free of charge. Due to space limitation (maximum of 12 participants), registration is mandatory. For more information, please visit the course website.

Program 2017
Presentation 1 - Digital images - 32 slides
Presentation 2 - Using Fiji - 29 slides
Exercise booklet
Lecture series for graduate students

This course is intended for Master and Ph.D. students with an interest in the structure and function of the cell nucleus. Although the molecular mechanisms at work in the nucleus are alluded to throughout the course, the emphasis is clearly placed on the cell biological aspects of this key organelle. These aspects are illustrated with recent experimental results taken from the literature, in particular those obtained through observation of single living cells. Broad explanatory concepts are preferred over the description of molecular details. The goal of the course is to present the cell nucleus as a dynamic biological system that is responsible for the expression, the repair and the replication of the genetic information. The integration of these functions within the global cellular context will also be presented. (All downloadable files are in PDF format.)

Syllabus 2017
Schedule 2017
Examination - Examples of questions
Lecture 1 - Origin and evolution of the cell nucleus - 77 slides
Lecture 1 - Recommended reading
Lecture 2 - Concepts and techniques - 49 slides
Lecture 2 - Recommended reading
Lecture 3 - Chromatin - 63 slides
Lecture 3 - Recommended reading
Lecture 4 - Nuclear periphery - 71 slides
Lecture 4 - Recommended reading
Lecture 5 - Nuclear bodies - 64 slides
Lecture 5 - Recommended reading
Lecture 6 - Chromosome territories - 79 slides
Lecture 6 - Recommended reading
Lecture 7 - Spatial gene positioning - 46 slides
Lecture 7 - Recommended reading
Lecture 8 - DNA replication and repair - 49 slides
Lecture 8 - Recommended reading
Lecture 9 - Transcription - 45 slides
Lecture 9 - Recommended reading
Lecture 10 - Nuclear dynamics - 64 slides
Lecture 10 - Recommended reading
Lecture 11 - Biophysics of the cell nucleus - 29 slides
Lecture 11 - Recommended reading
Cell cycle and gene expression
Basics of gene expression for first year medical students

What exactly do we mean when we say that someone has genes "for" brown eyes? A lot actually. The road from DNA molecule to brown eyes, or any other phenotype for that matter, is a complex one. The goal of this undergraduate-level lecture is to present its conceptual and mechanistic outlines. The presentation covers the main steps of gene expression, i.e. transcription, RNA processing, RNA splicing, and translation, as well as some of the means through which each of these steps is regulated. The first part of the lecture describes DNA replication and the cell cycle. This lecture was given to students of the First Faculty of Medicine at Charles University in Prague in January 2010.