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HATCHING OF C. ELEGANS LARVAE
12.5 hours - 3.3min interval - 9 fps - 4.11Mb

The entire development occurs in an eggshell without any growth. The embryo starts to move while still enclosed in the eggshell. After hatching, the L1 larvae consume the surrounding bacteria.

GROWTH OF C. ELEGANS
72.2 hours - 26.6min interval - 9 fps - 4.53Mb

Growth of C. elegans amounts to the partial transformation of bacteria into worms! Each adult hermaphrodite lays between 200-300 eggs. After hatching, the worm passes through 4 larval stages.

UNCOORDINATED WORMS
2 hours - 1.6min interval - 8 fps - 0.84Mb

Movement of the worm requires the alternate contraction of the dorsal and ventral muscles. Various mutations lead to uncoordination of these contractions. These unc mutants display greatly reduced motility. Shown in this movie is the EG6699 unc-119 mutant.

GROWTH OF MOUSE EMBRYONIC STEM CELLS
64.5 hours - 30 min interval - 6 fps - 4.79Mb

Mouse embryonic stem cells grow as colonies on a feeder of inactivated embryonic fibroblasts. Growth is rapid and individual cells are highly plastic, as shown in this video of DIC images captured on a Leica SP5 microscope at 30-minute intervals. Cells tend to continue moving within colonies. Note that to facilitate observation the cells were seeded sparsely (which is not usually done because it is detrimental to the maintenance of pluripotency) and that the feeder layer is anything but static!

BELOUSOV-ZHABOTINSKY REACTION
2 min 23 - 1 second interval - 10 fps - 1.96Mb

Arguably the most famous oscillating chemical reaction! Mix malonic acid, potassium bromide, potassium bromate in strongly acidic conditions, add a redox indicator (ferroin, red color) and observe the repeated but transient appearance of the oxydized form of the indicator (blue color). This reaction occurs far from the themodynamic equilibrium, like most reactions in living organisms. For details, please see here. I have performed this experiment because I am greatly interested by patterning in nonlinear systems and also because the pulsatile nature of the reaction reminds me of transcriptional pulsing...

EARLY DEVELOPMENT OF C. ELEGANS
1.5 hour - 2 minutes interval - 6 fps - 0.79Mb

The C. elegans embryo develops according to invariant cell lineages. The movie shows the early development of a worm line expressing a membrane-targeted GFP and a histone H2B-cherry fusion protein (strain OD95), from the zygote to the approx. 20-25-cell stage. Time is indicated as hour:minute:second, with 00:00:00 set at the time of fusion of the maternal and paternal pronuclei.